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Total time 14m Step 1 of 4 ยท Substep 1 of 2

Protocol For Phusion Hot Start Flex 2x Master Mix M0536

Substeps: 2

  • 1. 12.5 uL: Phusion Hot Start Flex Master Mix tube -> PCR well A1 Queued
  • 2. 2.5 uL: Primer/template tubes -> PCR well A1 Queued

Substeps: 1

  • 1. 25 uL: Bench setup block -> Thermocycler block Queued

Substeps: 1

  • 1. 5 uL: PCR well A1 -> Gel lane 1 Queued