Experiment notebook
Golden Gate Assembly Test
Hypothesis
Lower induction temperature should improve soluble protein yield without extending the run beyond a single shift.
Plan
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Prepare media
37 C · 15 min
Prepare media
37 C · 15 min
Add inducer
IPTG · 1 mM
Incubate culture
220 rpm · 4 h
Runner
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Prepare media
queued
Add inducer
queued
Incubate culture
queued
Results
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